Antiphotoaging effects of light-emitting diode irradiation on narrow-band UVB-exposed cultured human skin cells.

Type:Uv phototherapy   Time:2014-12-03 15:22:07
Antiphotoaging effects of light-emitting diode irradiation on narrow-band UVB-exposed cultured human skin cells.
Tian YS1, Kim NH, Lee AY.
Author information
Abstract
BACKGROUND:
Antiaging effects of light-emitting diodes (LEDs) have been clinically demonstrated using one or two wavelengths. The mechanism is unclear.
OBJECTIVE:
To examine direct and indirect photobiomodulation effects of LEDs on narrow-band ultraviolet B (NB-UVB)-induced photoaging using seven different wavelengths alone or in combination.
MATERIALS AND METHODS:
Four LED wavelengths were chosen based on type I collagen and metalloproteinase (MMP)-1 expression. NB-UVB-irradiated fibroblasts or keratinocytes were irradiated using these four wavelengths. The expression of collagen and MMP-1 in fibroblasts with or without conditioned medium from LED-irradiated keratinocytes and the expression of proinflammatory cytokines in the LED-irradiated keratinocytes were examined.
RESULTS:
Irradiation with four wavelengths (630, 660, 830, and 850 nm) significantly increased the number of viable fibroblasts. These four wavelengths also increased type I collagen expression, particularly four combinations (630/830, 660/850, 630/850, and 660/830 nm). The fibroblasts cultured with the keratinocyte conditioned medium, particularly with a combination of 630/850 or 660/830 nm, increased collagen levels. Low tumor necrosis factor alpha (TNF-¦Á) and high transforming growth factor beta 1 (TGF-¦Â1) expression was detected in the LED-irradiated keratinocytes.
CONCLUSION:
The combination of 630/850- or 660/830-nm irradiation led to better direct and indirect antiphotoaging outcomes than single LED wavelengths in NB-UVB-irradiated cultured normal human skin cells.
www.kerneluvb.com----The leader manufacturer of UV Phototherapy.