UVB therapy decreases the adhesive interaction between peripheral blood mononuclear cells and dermal microvascular endothelium, and regulates the differential expression of CD54, VCAM-1, and E-selectin in psoriatic plaques.

Type:Uv phototherapy   Time:2017-11-01 11:20:23
UVB therapy decreases the adhesive interaction between peripheral blood mononuclear cells and dermal microvascular 
endothelium, and regulates the differential expression of CD54, VCAM-1, and E-selectin in psoriatic plaques.
Cai JP1, Harris K, Falanga V, Taylor JR, Chin YH.
Author information
Abstract
A dermal lymphocytic infiltrate is a characteristic feature of psoriasis, and may be involved in the pathogenesis of 
the disease. We have previously shown that specialized dermal microvascular endothelial cells (DMEC) in psoriatic 
lesions promote the selective adherence of the CD4 CD45Ro helper T-cell subset. In this study, we examined the 
adhesive interaction between peripheral blood mononuclear cells and psoriatic DMEC in patients treated with 
ultraviolet B light (UVB), and correlated the results with the expression and function of endothelial adhesion 
molecules on DMEC. Seven psoriatic patients were exposed to one MED of UVB daily for 14 days, and the binding 
properties of their peripheral blood mononuclear cells (PBMC), and tissue specimens taken from their lesions on days 
0, 2, 3, 6, 8, 11 and 14 of UVB treatment, were studied. The ability of psoriatic PBMC to adhere to non-irradiated 
control or UVB-treated psoriatic plaques was reduced by 70% after treatment with 2-3 MED, and complete inhibition was 
obtained after 8-11 MED. In contrast, exposure of psoriatic plaques to 2-3 MED had no effect on the capacity of DMEC 
to support normal PBMC binding, which was only reduced after 8-11 MED. In addition, psoriatic plaques which were 
shielded from direct UVB exposure also showed decreased PBMC binding, suggesting a systemic effect of UVB treatment. 
Immunoperoxidase staining revealed that CD54 (ICAM-1) and E-selectin were strongly expressed on dermal vessels in 
untreated psoriatic plaques. Treatment of patients with 6-8 MED significantly decreased CD54 and E-selectin 
expression. In contrast, VCAM-1 expression on untreated plaques was weaker than that of CD54 and E-selectin, but was 
markedly induced following UVB treatment. In functional blocking studies, preincubation of tissue from untreated 
psoriatic plaques with anti-E-selectin antibody, but not antibodies against CD54 and VCAM-1, significantly inhibited 
the ability to bind normal PBMC. These observations suggest that UVB treatment interferes with the adhesive properties 
of both psoriatic PBMC and endothelial cells, and differentially regulates the expression of endothelial adhesion 
molecules. The study also provided direct evidence for the involvement of E-selectin in the adhesion of circulating 
lymphocytes to psoriatic endothelial cells.
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