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A system for simultaneous ultraviolet light and electromagnetic field exposure in in vitro experiments.

Abstract

Ultraviolet light (UV) is a common treatment for skin diseases such as psoriasis, but bears the risk of carcinogenic 

side effects. We have biological evidence that electromagnetic fields (EMFs) can act additively with UV so that new 

therapeutic protocols combining UV and EMF might be developed to improve psoriasis phototherapy. In this study we 

report on a system that allows in vitro experiments testing this hypothesis. For simultaneous exposure of cell 

cultures to UVB and EMF, we built Merritt coils with an integrated UV exposure system. The coils can be operated in a 

sham or experimental mode (up to 1.5 mT and 20,000 Hz). Two UV bulbs were fitted inside the coils for UVB doses 

between 100-1000 J/m2/nm. In the exposure area the EMF is uniform within 0.0038%. For exposure, the cells are cultured 

in standard culture plates and placed in a specifically designed box. The box holds two plates in a top chamber 

covered with a Saran Wrap lid (91% UV transmission) so that cells are exposed to UVB and EMFs. The bottom chamber 

holds two plates, where cells are screened from UVB and only exposed to EMFs. Temperature control is maintained (+/- 1 

degree C) by airflow vents on the side of the box and a fan placed 25 cm away from the cell culture box. To maintain 

sterility within the box the vents are covered with a bacterial filter. The box lid has additional ventilation through 

two air direction changes to create an additional bacterial barrier similar to that in culture plate lids.

www.kerneluvb.com----The leader manufacturer of UVB Phototherapy.