UVB-activated psoralen reduces luminal narrowing after balloon dilation because of inhibition of constrictive remodeling.
Type:Uv phototherapy Time:2017-09-25 14:32:12UVB-activated psoralen reduces luminal narrowing after balloon dilation because of inhibition of constrictive
remodeling.
Perr��e J1, van Leeuwen TG, Velema E, Smeets M, de Kleijn D, Borst C.
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Abstract
In this study we have explored the potential of PUVB (8-MOP + UVB) therapy for the reduction of luminal narrowing
after arterial injury. In 15 rabbits, balloon dilation of iliac arteries was performed. In 20 arteries, dilation was
combined with the delivery of pulsed ultraviolet light B (UVB) irradiation with 10 arteries being previously subjected
to sensitizer infusion. Changes in vessel diameter, proliferation and extracellular matrix protein content at 6 weeks
were evaluated by means of angiography and histomorphometry-immunohistochemistry. We found that PUVB, applied at the
time of dilation, induced reduction in late loss (LL) at 6 weeks (percutaneous transluminal angioplasty vs UVB vs
PUVB: 0.64 +/- 0.15 mm vs 0.61 +/- 0.05 mm vs 0.29 +/- 0.05 mm; p = 0.018). The same holds true for constrictive
remodeling (0.53 +/- 0.15 mm vs 0.45 +/- 0.06 mm vs 0.15 +/- 0.05 mm; p = 0.016). In the irradiation groups, LL was
independent of acute gain (AG), as opposed to the control. Collagen content increased significantly after PUVB in
media and adventitia, without increased cellular proliferation in all vessel layers. Thus, PUVB at the time of
dilation reduced luminal narrowing at follow-up without effecting proliferation. This effect was independent of AG and
was associated with increased collagen content in media and adventitia.
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